ACTIVITY OF IMMOBILIZED LIPOXYGENASE USED FOR THE FORMATION OF PERHYDROXYACIDS

Lipoxygenase-produced oxidized fatty acids may serve as intermediates in chemical and pharmaceutical syntheses. For practical applications t he enzyme should be immobilized to prevent its loss at product retriev al and to enable a continuous process. In this study the immobilizatio n of soybean lipoxygenase on various neutral and charged supports was investigated. The best results were obtained using cellulose-based ani on exchangers. The activity of the immobilized lipoxygenase was lower than the activity of the free enzyme, which was possibly caused by dif fusion limitation of the fatty acids to or from the support beads.