INFLUENCE OF ENDOTHELIAL NITRIC-OXIDE ON NEUROGENIC CONTRACTION OF HUMAN PULMONARY-ARTERIES

The present study was designed to investigate the contribution of the endothelium and that of the L-arginine pathway on the contractile resp onses of isolated human pulmonary arteries to electrical field stimula tion (EFS) and noradrenaline. Isometric tension was measured in artery rings obtained from portions of human lung after thoracic surgery for removal of lung carcinoma (18 patients). Electrical field stimulation (EFS) induced frequency-dependent contractions of isolated human pulm onary arteries which were abolished by tetrodotoxin, guanethidine and prazosin (all at 10(-6) M). The increases in tension were of greater m agnitude in arteries denuded of endothelium. N-G-nitro-L-arginine meth yl ester (L-NAME) (10(-4) M) potentiated the contractile response to E FS in artery rings with endothelium but not in endothelium-denuded art eries, The potentiation induced by L-NAME was completely reversed by L -arginine (10(-4) M) but not by D-arginine (10(-4) M), Indomethacin (3 x 10(-6) M) had no significant effect on the contractile response to EFS, Contractile responses to noradrenaline were similar in arteries w ith and without endothelium. Our results suggest that electrical field stimulation releases endothelium-derived nitric oxide, which inhibits the contractile responses of human pulmonary arteries. Although adren ergic nerves seem to be responsible for the contraction, the transmitt er involved in the release of nitric oxide does not appear to be norad renaline.