STRUCTURAL-PROPERTIES OF RIBOSOMAL-PROTEI N S8 FROM THE EXTREME THERMOPHILE THERMUS-THERMOPHILUS

Authors
VYSOTSKAYA VS NASIBULLIN UF UVERSKII VN VASILENKO KS NARIZHNEVA NV GARBER MB
Citation
Vs. Vysotskaya et al., STRUCTURAL-PROPERTIES OF RIBOSOMAL-PROTEI N S8 FROM THE EXTREME THERMOPHILE THERMUS-THERMOPHILUS, Bioorganiceskaa himia, 21(7), 1995, pp. 492-497
Citations number
38
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
21
Issue
7
Year of publication
1995
Pages
492 - 497
Database
ISI
SICI code
0132-3423(1995)21:7<492:SORNSF>2.0.ZU;2-X
Abstract
The gene of ribosomal protein Ss from the extreme thermophile Thermus thermophilus was expressed in E. coil using the strain BL21(DE3) and v ector pET3-1. A method of isolating this protein from the superproduci ng strain was developed, which makes it possible to obtain 8 - 12 mg o f product from 11 of culture. The secondary structure of protein S8 wa s determined by using CD spectroscopy. The protein was shown to be hig hly resistant to denaturants.