Eradication of poliomyelitis is based on the mass administration of or
al poliovirus vaccine (OPV). Delivery of effective vaccines in the dev
eloping world especially in tropical areas, is compromised when refrig
eration cannot be assured The OPV, prepared with three live attenuated
polioviruses (Sabin strains, serotypes 1, 2 and 3), is considered to
be the most thermolabile of vaccines in the World Health Organization'
s Expanded Programme on Immunization. To be effective, the initial con
centration (potency) of each of the three component serotypes, measure
d in tissue culture infective closes, should not decrease by more than
0.5 log(10) before vaccine delivery. High concentration (1 M) of MgCl
2 is currently used as stabilizer for OPV. The stabilizing effect of D
2O was tested here on OPV strains. By diluting the viral suspension wi
th D2O-based salt and buffer solutions, in a manner similar to that in
volved in OPV production, an 87% concentration of D2O in the final vir
al preparation was achieved. In severe conditions of testing (incubati
on for 3 days at 45 degrees C), the Sabin 3 virus lost an average of 2
.7 log(10) potency in the presence of 87% D2O as compared to 3.0 log(1
0) in H2O-based IM MgCl2, and to 5.7 log(10) in the H2O-based control
solutions when tested in a combined 87% D2O and 1 M MgCl2, treatment,
the Sabin 3 virus lost only 1.3 log(10) potency after 3 days cat 45 de
grees C. Similar thermostabilizing tlffects were obtained for Sabin 1
and Sabin 2 strains, but the level of stabilization was slightly lower
Tested in standard conditions at 37 degrees C for 7 days, the infecti
vity of the three D2O MgCl2-treated OPV strains remained in the limit
of requirements (less than or equal to 0.5 log(10)). The stabilizing e
ffect of D2O was also demonstrated on yellowfever 170 vaccine virus st
rain. D2O alone or in combination with other stabilizers such as MgCl2
, could thus be considered as a candidate for the improvement of live
virus vaccine thermostability.