SIMULTANEOUS IMMUNOAFFINITY PURIFICATION OF O-6-METHYLGUANINE, O-6-ETHYLGUANINE, O-6-PROPYLGUANINE AND O-6-BUTYLGUANINE AND THEIR ANALYSIS BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

Authors
AIROLDI L MAGAGNOTTI C CHIAPPETTA L BONFANTI M PASTORELLI R
Citation
L. Airoldi et al., SIMULTANEOUS IMMUNOAFFINITY PURIFICATION OF O-6-METHYLGUANINE, O-6-ETHYLGUANINE, O-6-PROPYLGUANINE AND O-6-BUTYLGUANINE AND THEIR ANALYSIS BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY, Carcinogenesis, 16(9), 1995, pp. 2247-2250
Citations number
16
Categorie Soggetti
Oncology
Journal title
Carcinogenesis → ACNP
ISSN journal
01433334
Volume
16
Issue
9
Year of publication
1995
Pages
2247 - 2250
Database
ISI
SICI code
0143-3334(1995)16:9<2247:SIPOOO>2.0.ZU;2-Z
Abstract
A sensitive and specific method has been developed for the simultaneou s analysis of different O-6-alkylguanines. The cross-reactivity of two different antibodies raised against O-6-methylguanosine and O-6-butyl guanosine for a series of O-6-alkylguanines was exploited for the immu noaffinity purification of biological samples before quantitative anal ysis by gas chromatography/mass spectrometry. The method can be applie d to the detection of O-6-alkylguanines in DNA and appears to be usefu l for studying chemical carcinogen mechanisms in animals and possibly for the detection of human exposure to alkylating agents.