IDENTIFICATION OF YEASTS BY HYDROLYSIS OF AMIDES

Citation
J. Miragutierrez et al., IDENTIFICATION OF YEASTS BY HYDROLYSIS OF AMIDES, Mycoses, 38(3-4), 1995, pp. 101-106
Citations number
20
Categorie Soggetti
Dermatology & Venereal Diseases",Mycology
Journal title
ISSN journal
09337407
Volume
38
Issue
3-4
Year of publication
1995
Pages
101 - 106
Database
ISI
SICI code
0933-7407(1995)38:3-4<101:IOYBHO>2.0.ZU;2-6
Abstract
We analysed the hydrolysis of 23 amides by 500 yeast and yeast-like st rains isolated from clinical specimens, identified to species level by conventional methods, in order to verify the validity of this method of species identification. The results show that 10 of these amides (a cetamide, acrylamide, alaninamide, formamide, glycinamide, propionamid e, urea, thioacetamide, thiourea and valeramide) are sufficient to dif ferentiate seven genera and 19 species, with an occasional requirement for three additional tests: cycloheximide susceptibility, surface fil m formation on liquid medium and ascospore formation. The study of the amidase activity in yeasts and yeast-like fungi seems to be a promisi ng method of identifying strains isolated from clinical samples.