MOLECULAR-CLONING AND SEQUENCING OF THE GENE ENCODING A SHEEP ARGININE-VASOPRESSIN TYPE 1A RECEPTOR

The gene for the sheep arginine vasopressin type 1a (V-1a) receptor su btype was cloned from a genomic library. The deduced amino acid sequen ce shows characteristics of a G-protein coupled receptor and high sequ ence identity to human and rat V-1a receptor sequences (81% and 73%, r espectively). Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed a tissue distribution consistent with a type V-1a rec eptor. The genomic DNA (7.1 kb) contains a 1586 bp intron between the putative 6th and 7th transmembrane domains,