PROMOTER ANALYSIS OF THE BOVINE GENE FOR SEMINALPLASMIN

Authors
HERBERS J SCHEIT KH
Citation
J. Herbers et Kh. Scheit, PROMOTER ANALYSIS OF THE BOVINE GENE FOR SEMINALPLASMIN, Biochimica et biophysica acta, N. Gene structure and expression, 1263(3), 1995, pp. 271-274
Citations number
22
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1263
Issue
3
Year of publication
1995
Pages
271 - 274
Database
ISI
SICI code
0167-4781(1995)1263:3<271:PAOTBG>2.0.ZU;2-V
Abstract
In this study we mapped the transcriptional initiation site of the gen e for seminalplasmin (SAP) by primer extension analysis, situated 125 nucleotides upstream of the translational initiation site of the SAP-s pecific mRNA. We showed that the TATA-box in position -30 of the SAP g ene is part of a functional promoter. A 280 bp region of the 5'-flanki ng region exerted a strong positive effect on promoter activity. In th is region we identified consensus sequences for the transcriptional co ntrol elements API, AP2, PEA3 and GATA.