IMMUNITY TO ST-LOUIS ENCEPHALITIS-VIRUS BY SEQUENTIAL IMMUNIZATION WITH RECOMBINANT VACCINIA AND BACULOVIRUS DERIVED PRM E PROTEINS/

Citation
K. Venugopal et al., IMMUNITY TO ST-LOUIS ENCEPHALITIS-VIRUS BY SEQUENTIAL IMMUNIZATION WITH RECOMBINANT VACCINIA AND BACULOVIRUS DERIVED PRM E PROTEINS/, Vaccine, 13(11), 1995, pp. 1000-1005
Citations number
23
Categorie Soggetti
Immunology
Journal title
ISSN journal
0264410X
Volume
13
Issue
11
Year of publication
1995
Pages
1000 - 1005
Database
ISI
SICI code
0264-410X(1995)13:11<1000:ITSEBS>2.0.ZU;2-S
Abstract
St. Louis encephalitis (SLE) is an important mosquito-borne disease of great public health concern in parts of the United States, South Amer ica and Canada. Protective immunogens of flaviviruses produced in diff erent expression systems have been shown to be effective against virul ent virus infection in laboratory animal models. Here we show that the pre-membrane and envelope (PrM/E) of SLE virus expressed in insect an d mammalian cell systems using baculovirus and vaccinia virus, respect ively, are processed correctly and showed similar antigenic characteri stics as the authentic proteins, Immunization with the recombinant pro teins individually or in combination resulted in neutralizing and prot ective immune responses. A schedule consisting of initial immunization with recombinant vaccinia virus followed by a secondary boost with re combinant baculovirus protein resulted in higher levels of neutralizin g and protective immune responses. The advantages of the use of such a combined approach as a general immunization strategy are discussed.