CHARACTERIZATION, IN-VITRO AND IN-VIVO STUDIES ON PRIMAQUINE DIPHOSPHATE LIPOSOMES

In this study, several Primaquine diphosphate (PQ) liposomal formulati ons containing phospholipid, charge inducer and with or without choles terol in molar ratios of 7:1:(2) and 10:1:(4) were investigated. Gel s tate (DPPC:CHEMS:CHOL and PL-100H:CHEMS:CHOL) and liquid-crystalline s tate (PL-100:CHEMS:CHOL and PL-90G:CHEMS:CHOL) liposomes were prepared . The film method followed by sonication and extrusion through polycar bonate membrane was used. Particle size distribution, percentage of en trapped active substance, content of phospholipid and bilayer type and composition were determined. Lamellarity was determined by P-31-NMR t echnique. In vitro release of PQ was investigated at 37 degrees C, 35 rpm and in Tris (pH: 7.4) buffer. In vitro release and its fit to kine tic models were investigated. Liposomes were labelled by Tc-99m and in jected intravenously to Swiss Albino mice.