S. Latkovic et Seg. Nilsson, ULTRASTRUCTURAL VISUALIZATION OF LECTIN RECEPTORS IN NORMAL AND INJURED EPITHELIUM OF THE RABBIT CORNEA, Optometry and vision science, 72(9), 1995, pp. 643-648
Lectin receptors of the rabbit corneal epithelium were investigated ul
trastructurally using gold-conjugated lectins. Corneal epithelium with
an intact mucous layer was readily labeled with wheat-germ agglutinin
(WGA), whereas after mechanical or chemical removal of the mucus, lab
eling was minimal, suggesting that the lectin receptors were located m
ainly in the mucus. Corneal epithelium subjected to slight superficial
injury was also labeled with WGA. In this case, however, the gold par
ticles were in close contact with the cell membrane of injured and/or
newly exposed cells. In the more injured cells, gold particles were se
en in the cytoplasm as well. Labeling with WGA indicates the presence
of sialyl residues, known to be attachment sites for Pseudomonas aerug
inosa. The results suggest a protective role of mucus against infectio
n. The association of WGA lectin receptors with the plasma membrane of
compromised corneal epithelial cells may help to explain the mechanis
m of bacterial invasion of the cornea, e.g., in overnight wear of cont
act lenses with insufficient oxygen transmissibility.