PULSED-FIELD GEL-ELECTROPHORESIS COMBINED WITH RARE-CUTTING ENDONUCLEASES FOR STRAIN DIFFERENTIATION OF CANDIDA-FAMATA, KLOECKERA-APICULATAAND SCHIZOSACCHAROMYCES-POMBE WITH CHROMOSOME-NUMBER AND SIZE ESTIMATION OF THE 2 FORMER

Transverse alternating field electrophoresis combined with digestion o f chromosomal DNAs by rare-cutting restriction endonucleases as Not I, Sfi I and Sma I was proved to be efficient for the differentiation of several strains of the yeasts Schizosaccharomyces pombe, Kloeckera ap iculata and Candida famata. For these two last genera the chromosome n umber, estimated from electrophoretic karyotypes, varied within the sa me species from six to eight. The total size of the genome was next to 10 Mpb for KI. apiculata and from 14 to 12 Mpb for C. famata.