PRODUCTION OF HIGH-CONTENT FRUCTO-OLIGOSACCHARIDES BY AN ENZYMATIC SYSTEM FROM PENICILLIUM-RUGULOSUM

The production of high-content fructo-oligosaccharides from sucrose by a crude FTF from a new strain of Penicillium isolated in our Laborato ry was investigated. The optimum conditions for the production of the enzyme acid for the enzymic reaction have been determined. It has been demonstrated that the crude enzyme acts as a mixed enzyme system of f ructosyl transferase (FTF; Class 2 of Enzyme Nomenclature) and glycosi dases (Class 3 of Enyme Nomenclature), Under optimum conditions: pH 5. 5, temperature 55 degrees C, sucrose concentration 750 g/l, enzyme con centration 5 FTF units/g sucrose; conversion yield up to 80%, were obt ained and high concentration of nystose (412 g/l) and fructofuranosyl- nystose (176 g/l) were accumulated.