HIGH-LEVEL PRODUCTION OF RECOMBINANT INVERTASE IN HANSENULA-POLYMORPHA

Authors
NARCIANDI RE RODRIGUEZ L RODRIGUEZ E DIAZ R DELGADO J HERRERA L
Citation
Re. Narciandi et al., HIGH-LEVEL PRODUCTION OF RECOMBINANT INVERTASE IN HANSENULA-POLYMORPHA, Biotechnology letters, 17(9), 1995, pp. 949-952
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
9
Year of publication
1995
Pages
949 - 952
Database
ISI
SICI code
0141-5492(1995)17:9<949:HPORII>2.0.ZU;2-7
Abstract
The SUC 2 gene from Saccharomyces cerevisiae coding for the enzyme Inv ertase was cloned in Hansenula polymorpha under the control of the alc ohol-oxidase (AOX) promoter of Pichia pastoris. The culture conditions for the Invertase production using a fed-batch culture were studied. More than 1,5 x 10(3) U/ml of Invertase (1 g/L) were found to be secre ted to cellular periplasmic space. The scale up to 50 L of the ferment ative process was done.