THERMOSTABILIZATION OF LACTATE OXIDASE BY RANDOM MUTAGENESIS

Authors
MINAGAWA H NAKAYAMA N NAKAMOTO S
Citation
H. Minagawa et al., THERMOSTABILIZATION OF LACTATE OXIDASE BY RANDOM MUTAGENESIS, Biotechnology letters, 17(9), 1995, pp. 975-980
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
9
Year of publication
1995
Pages
975 - 980
Database
ISI
SICI code
0141-5492(1995)17:9<975:TOLOBR>2.0.ZU;2-M
Abstract
A lactate oxidase (LOD) gene from Aerococcus viridans was cloned and s equenced to generate thermostable LOD, One mutant LOD selected from a set of variants created by random mutagenesis had a half life of 6.2 m in at 65 degrees C, approximately three times longer than that of the wild type LOD. This mutant exhibited an Asn to Asp point mutation at p osition 212 in the amino acid sequence.