Lrm. Marques et al., ASSOCIATION BETWEEN ALPHA-HEMOLYSIN PRODUCTION AND HELA CELL-DETACHING ACTIVITY IN FECAL ISOLATES OF ESCHERICHIA-COLI, Journal of clinical microbiology, 33(10), 1995, pp. 2707-2709
Escherichia coli isolates that cause detachment of cell monolayers dur
ing in vitro adherence assays (cell-detaching E. coli [CDEC]) were rec
ently reported as a potential new group of enteropathogenic bacteria.
In the present study, 269 E. coli isolates from feces of children 1 to
5 years of age were identified as CDEC in a detaching assay developed
with HeLa cells. The great majority of these isolates,were hemolytic,
within 3 h of growth on blood agar plates and hybridized with a DNA pr
obe for alpha-hemolysin (93.7%), while most of the non-detaching isola
tes were hemolytic,within 24 h (3.6%) or nonhemolytic (94.8%). E. coli
isolates that produced alpha hemolysin were found in 60 (30%) of 200
children with diarrhea and 47 (24%) of 200 age-matched controls. No st
atistical significance was found for the differences in alpha-hemolysi
n production among the matched pairs (P = 0.2). These data suggest tha
t CDEC isolates are not associated with diarrhea in the population stu
died.