NONISOTOPIC HYBRIDIZATION ASSAY FOR DETERMINATION OF RELATIVE AMOUNTSOF GENOTYPIC HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ZIDOVUDINE RESISTANCE

Authors
EASTMAN PS BOYER E MOLE L KOLBERG J URDEA M HOLODNIY M
Citation
Ps. Eastman et al., NONISOTOPIC HYBRIDIZATION ASSAY FOR DETERMINATION OF RELATIVE AMOUNTSOF GENOTYPIC HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ZIDOVUDINE RESISTANCE, Journal of clinical microbiology, 33(10), 1995, pp. 2777-2780
Citations number
15
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
10
Year of publication
1995
Pages
2777 - 2780
Database
ISI
SICI code
0095-1137(1995)33:10<2777:NHAFDO>2.0.ZU;2-W
Abstract
A nonisotopic hybridization assay for human immunodeficiency virus gen otypic zidovudine resistance determination is described. Biotinylated PCR product was hybridized with enzyme labeled probes for,wild-type or resistant mutant sequences and detected colorimetrically or chemilumi nescently in a microplate format. Changes in mutant-to-wild-type ratio s allow for the monitoring of longitudinal patient samples.