CHROMATOGRAPHIC TECHNIQUES FOR THE ISOLATION AND PURIFICATION OF LIPOPROTEINS

Various modes of chromatography are available for lipoprotein separati on. Gel permeation and affinity chromatography are used for preparativ e purposes and to separate lipoproteins according to size and apolipop rotein content, respectively. Development of rigid supports for gel pe rmeation has led to large improvements in speed and resolution. Revers ed-phase high-performance liquid chromatography (HPLC) of apolipoprote ins offers the best performance in terms of speed and resolution of st ructural variants. Due to its high speed and superior resolving power, the recently developed technique of capillary electrophoresis should emerge as an important method for lipoprotein analysis.