HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PHOSPHATIDIC-ACID

This paper reviews existing high-performance liquid chromatographic (H PLC) methods for the analysis of phosphatidic acid (PA) in various sam ple matrices. In addition to the introductory background discussion on important aspects of PA in lipid biochemistry, the review provides co mprehensive coverage in the areas of derivatization techniques, detect ion methods, and HPLC separation techniques. Conversions of PA to suit able derivatives enhance the detection sensitivity and improve the chr omatographic behavior of the analytes. Detection methods include the u se of state-of-the-art detectors and are discussed in terms of sensiti vity, specificity, and compatibility with analytical systems. Pertinen t normal-phase and reversed-phase HPLC data for PA are compiled from p ublished methods.