Because lethal heat shocks perturb a multitude of cellular processes,
the primary lesions responsible for death from heat stress remain to b
e defined. In Drosophila, sublethal heat treatments produce developmen
tal anomalies that frequently mimic the effects of known mutations and
are hence referred to as phenocopies. Mutations subject to phenocopy
mimicry provide signposts to those biological processes most sensitive
to heat and most important for the function and survival of the organ
ism as a whole. We have analyzed a particular developmental defect ind
ucible in early embryos of Drosophila melanogaster. By molecular, phen
otypic, and genetic criteria, we have found extensive parallels betwee
n this phenocopy and certain dominant mutations in the segmentation ge
ne fushi tarazu (ftz). Our analysis of this phenocopy indicates that t
he crucial lesion is interference with proper turnover of ftz protein,
resulting in ftz overexpression. Our results provide a novel explanat
ion for a heat-induced developmental defect. Perturbations in relative
amounts of important regulatory proteins may be a common mechanism by
which heat-shock phenocopies arise.