ENHANCEMENT OF MUTATION FREQUENCY WITH NUCLEOTIDE TRIPHOSPHATE ANALOGS IN PCR RANDOM MUTAGENESIS

Authors
UEDA H NAKANISHI M SUZUKI E NAGAMUNE T
Citation
H. Ueda et al., ENHANCEMENT OF MUTATION FREQUENCY WITH NUCLEOTIDE TRIPHOSPHATE ANALOGS IN PCR RANDOM MUTAGENESIS, Journal of fermentation and bioengineering, 79(3), 1995, pp. 303-305
Citations number
8
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
Journal title
Journal of fermentation and bioengineering → ACNP
ISSN journal
0922338X
Volume
79
Issue
3
Year of publication
1995
Pages
303 - 305
Database
ISI
SICI code
0922-338X(1995)79:3<303:EOMFWN>2.0.ZU;2-N
Abstract
The effects of the deoxynucleotide triphosphate analogs 2'-deoxyinosin e 5'-triphosphate and 5-bromo-2'-deoxyuridine 5'-triphosphate on the e nhancement of random mutation in PCR were investigated. Addition of th ese analogs was found to be an effective and easy method for making va rious mutant DNA sequences of lacZ'.