SALMONELLA STRAIN SECRETING ACTIVE LISTERIOLYSIN CHANGES ITS INTRACELLULAR-LOCALIZATION

Authors
GENTSCHEV I SOKOLOVIC Z MOLLENKOPF HJ HESS J KAUFMANN SHE KUHN M KROHNE GF GOEBEL M
Citation
I. Gentschev et al., SALMONELLA STRAIN SECRETING ACTIVE LISTERIOLYSIN CHANGES ITS INTRACELLULAR-LOCALIZATION, Infection and immunity, 63(10), 1995, pp. 4202-4205
Citations number
21
Categorie Soggetti
Immunology,"Infectious Diseases
Journal title
Infection and immunity → ACNP
ISSN journal
00199567
Volume
63
Issue
10
Year of publication
1995
Pages
4202 - 4205
Database
ISI
SICI code
0019-9567(1995)63:10<4202:SSSALC>2.0.ZU;2-V
Abstract
We describe the construction of an attenuated Salmonella dublin aroA s train which secretes via the Escherichia coli hemolysin secretion mach inery an active hybrid cytolysin consisting of listeriolysin from List eria monocytogenes and the C-terminal secretion signal of E. coli hemo lysin. This hemolytic S. dublin strain is partially released into the cytoplasm of the host cell following uptake by J774 macrophage cells, whereas the nonhemolytic control S. dublin aroA strain remains in the phagosome.