THE AUTOMATION OF IMMUN-FIA-SYSTEMS

In this contribution an automation system for the control, measurement uptake, evaluation and visualization is presented as well as its appl ication for two different flow-injection immunoassays. Both systems ar e based on the principles of now-injection analysis and were developed for a reliable, fast monitoring of relevant proteins in animal cell c ultivation processes. One system uses a homogeneous assay with turbidi metric detection, the other a heterogeneous assay with an immobilized immunocomponent applying fluorescence detection. The on-line applicati on during a mammalian cell cultivation as well as off-line measurement s of medium samples are presented. The results obtained from on-line m easurements are compared to ELISA results. The automation of the analy sis systems with respect to its flexible control as well as the reliab le evaluation of the measurement signals-even if they are severely dis torted-are discussed in detail.