A METHOD FOR THE ANALYSIS OF SOME NEUROLE PTICS AND CARAZOLOL IN PORKKIDNEYS

A method is described for the analytical determination of the neurolep tics azaperone (plus its metabolite azaperol), acetylpromazine, propio nylpromazine, chlorpromazine and the beta-blocking agent carazolol in pork kidneys. These compounds may be used illegally to calm pigs durin g their transport to the abattoir. The kidney samples (plus atosil as an internal standard) are incubated with NaOH (90 degrees C, 60 min); the rather fluid samples are extracted with diethylether. The separati on of interfering compounds in the extracts is achieved on a silica ge l column. The remaining interfering compounds are removed with ether a fter acidification of the eluted material. After alkalization of the a queous solution, the drugs are extracted with ether and applied to a S ymmetry RP18 column (mobile phase: acetate buffer solution pH 4.5/acet onitrile/tetrahydrofurane, 65/30/10 by volume by HPLC. Recovery in spi ked samples of pork kidneys (recovery of 50 mu g/kg, carazolol 5 mu g/ kg) was between 71.5% (for propionylpromazine) and 88% (for azaperol). The method was verified on samples of treated pigs.