APPLICABILITY OF THE PCR TECHNIQUE IN THE FOOD TESTING LABORATORY - IDENTIFICATION OF LISTERIA-MONOCYTOGENES

Authors
BLAIS BW PHILLIPPE LM BURZYNSKI M BIELECKI J
Citation
Bw. Blais et al., APPLICABILITY OF THE PCR TECHNIQUE IN THE FOOD TESTING LABORATORY - IDENTIFICATION OF LISTERIA-MONOCYTOGENES, Biotechnology techniques, 9(9), 1995, pp. 629-632
Citations number
6
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
9
Year of publication
1995
Pages
629 - 632
Database
ISI
SICI code
0951-208X(1995)9:9<629:AOTPTI>2.0.ZU;2-P
Abstract
A polymerase chain reaction (PCR) targeting Listeria monocytogenes-spe cific hlyA gene sequences was evaluated as a tool for the identificati on of presumptive positive colonies isolated on Palcam or Oxford agar during routine analysis of food samples by culture technique. The PCR correctly identified L monocytogenes isolates from a large number of s amples, and provided a significant saving in the time, labour and cost compared to the traditional biochemical tests.