The influence of two variables (cross-linking and protein concentratio
n) on the activity shown by alpha-amylase and invertase immobilized on
chitosan microbeads was studied by means of full factorial experiment
al designs. Microencapsulation on chitosan beads has shown to be an ef
fective immobilization method for both enzymes and observed difference
s in their behaviour are explained mainly by the molecular weight of t
heir substrates. Copyright (C) 1997 Elsevier Science Ltd.