Alc. Dornelles et al., SOMACLONAL VARIATION IN ALUMINUM TOLERANC E AND GIBBERELLIC-ACID SENSIBILITY IN WHEAT, Pesquisa agropecuaria brasileira, 32(2), 1997, pp. 193-200
In order to detect the induction of variability through tissue culture
and the utilization of somaclonal variation in wheat (Triticum aestiv
um L.) breeding, five genotypes of different statures and with toleran
ce to aluminum (Al+++) toxicity, and their F-1 hybrids, were submitted
to immature embryo culture. Parallel to the tissue culture, regenerat
ion and development processes, the genotypes and their progenies were
sown to obtain F-2 population, with or without tissue culture, which w
ere simultaneously tested by means of successive nutritive solutions i
n selective concentrations of Al+++ (10 ppm) and gibberellic acid-GA3
(100 ppm). The comparisons among populations submitted or not to tissu
e culture showed the existence of a possible somaclonal variation sinc
e some F-2 populations which passed through the in vitro process had a
differentiated behavior regarding the two evaluated traits. The metho
d employed confirmed the possibility of detecting genetic differences
in wheat and its possible utilization in plant breeding.