S. Karlsson et al., STIMULATION OF ENDOCRINE, BUT NOT EXOCRINE, PANCREATIC-SECRETION DURING 2-DEOXY-D-GLUCOSE-INDUCED NEUROGLYCOPENIA IN THE CONSCIOUS PIG, Pancreas, 11(3), 1995, pp. 271-275
The effects of autonomic nervous activation, initiated by 2-deoxy-D-gl
ucose (2-DG)-induced neuroglycopenia, on endocrine and exocrine pancre
atic secretion were investigated in the conscious pig. Pigs were surgi
cally fitted with permanent pancreatic duct and duodenal reentrant can
nulas, allowing long-term sampling of pancreatic juice, and a jugular
vein catheter for blood sampling and infusion of 2-DG. 2-DG was admini
stered as a 5-min intravenous infusion at three dose levels to conscio
us pigs. 2-DG (400 mg/kg) was found to elevate plasma glucagon and ins
ulin levels (p < 0.01). In contrast, exocrine pancreatic secretion, me
asured as volume, total protein output, and output of trypsin activity
was not affected by 2-DG at the dose levels of 75, 200, and 400 mg/kg
. Secretin (440 pmol/kg/h), however, stimulated pancreatic exocrine ou
tput of fluid (p < 0.01), protein (p < 0.01), and trypsin (p < 0.05).
It is concluded that antonomic nervous activation by 2-DG-induced neur
oglycopenia, in the conscious pig under basal conditions, elevates the
plasma levels of glucagon and insulin but does not affect exocrine pa
ncreatic secretion. 2-DG-induced neuroglycopenia is, thus, a suitable
model for studying autonomic neural influences on the porcine endocrin
e pancreas.