STIMULATION OF ENDOCRINE, BUT NOT EXOCRINE, PANCREATIC-SECRETION DURING 2-DEOXY-D-GLUCOSE-INDUCED NEUROGLYCOPENIA IN THE CONSCIOUS PIG

The effects of autonomic nervous activation, initiated by 2-deoxy-D-gl ucose (2-DG)-induced neuroglycopenia, on endocrine and exocrine pancre atic secretion were investigated in the conscious pig. Pigs were surgi cally fitted with permanent pancreatic duct and duodenal reentrant can nulas, allowing long-term sampling of pancreatic juice, and a jugular vein catheter for blood sampling and infusion of 2-DG. 2-DG was admini stered as a 5-min intravenous infusion at three dose levels to conscio us pigs. 2-DG (400 mg/kg) was found to elevate plasma glucagon and ins ulin levels (p < 0.01). In contrast, exocrine pancreatic secretion, me asured as volume, total protein output, and output of trypsin activity was not affected by 2-DG at the dose levels of 75, 200, and 400 mg/kg . Secretin (440 pmol/kg/h), however, stimulated pancreatic exocrine ou tput of fluid (p < 0.01), protein (p < 0.01), and trypsin (p < 0.05). It is concluded that antonomic nervous activation by 2-DG-induced neur oglycopenia, in the conscious pig under basal conditions, elevates the plasma levels of glucagon and insulin but does not affect exocrine pa ncreatic secretion. 2-DG-induced neuroglycopenia is, thus, a suitable model for studying autonomic neural influences on the porcine endocrin e pancreas.