V. Ganeva et al., FAST KINETIC-STUDIES OF PLASMID DNA TRANSFER IN INTACT YEAST-CELLS MEDIATED BY ELECTROPULSATION, Biochemical and biophysical research communications, 214(3), 1995, pp. 825-832
Intact yeast cell Electrotransformation process was investigated. It i
s a two step process. The plasmid must be pre-mixed and present in con
tact with the cells during the pulse. During the millisecond field pul
se, plasmid DNA is associated to the envelope. It therefore crosses th
e membrane by a process which lasts several seconds as shown by its se
nsitivity to a post pulse addition of DNase. Electrotransformation is
not supported by an electrophoretic transfer due to the external field
nor by a free diffusion across the electropermeabilized DNA is first
bound during the field pulse and then is transferred by a still unknow
n active process due to cell metabolism. (C) 1995 Academic Press, Inc.