EXPRESSION OF A HUMAN SERUM-ALBUMIN VARIANT WITH HIGH-AFFINITY FOR THYROXINE

In this study a protein expression system was used to synthesize recom binant human serum albumin containing a mutation that has been shown t o result in familial dysalbuminemic hyperthyroxinemia, Equilibrium dia lysis was used to measure the binding of this recombinant human serum albumin with thyroxine, The association constant determined for the bi nding of this human serum albumin variant with thyroxine was shown to be 65-fold greater than that of recombinant normal human serum albumin . (C) 1995 Academic Press, Inc.