ASSESSMENT OF GENETIC DIVERSITY AMONG STRAINS OF PSEUDOMONAS-SYRINGAEBY PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF RIBOSOMAL-RNA OPERONS WITH SPECIAL EMPHASIS ON P-SYRINGAE PV TOMATO
C. Manceau et A. Horvais, ASSESSMENT OF GENETIC DIVERSITY AMONG STRAINS OF PSEUDOMONAS-SYRINGAEBY PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF RIBOSOMAL-RNA OPERONS WITH SPECIAL EMPHASIS ON P-SYRINGAE PV TOMATO, Applied and environmental microbiology, 63(2), 1997, pp. 498-505
Phylogenetic relationships among 77 bacterial strains belonging to Pse
udomonas syringae and Pseudomonas viridiflava species were assessed by
analysis of the PCR-restriction fragment length polymorphism (RFLP) p
atterns of three DNA fragments corresponding to rrs and rrl genes and
the internal transcribed spacer, ITS1. No difference among all strains
in rrs and rrl genes was observed with 14 restriction enzymes, which
confirms the close relationships existing between these two species. T
he nucleotidic sequence of the internal transcripted spacer (ITS1) bet
ween rrs and rrl for the P. syringae pv. syringae strain CFBP1392 was
determined. Restriction maps of the PCR-amplified ITS1 region were pre
pared and compared for all 77 strains, Seventeen RFLP patterns, formin
g three main clusters, were distinguished, One contained all strains o
f P. syringae pv. tomato and of other pathovars which had been previou
sly described as closely related by either pathogenicity studies or bi
ochemical analyses. This cluster was equally far from P. viridiflava a
nd from other P. syringae pathovars, These other pathovars of P. syrin
gae formed a less coherent taxon.