Ki. Ueno et K. Shetty, EFFECT OF SELECTED POLYSACCHARIDE-PRODUCING SOIL BACTERIA ON HYPERHYDRICITY CONTROL IN OREGANO TISSUE-CULTURES, Applied and environmental microbiology, 63(2), 1997, pp. 767-770
Hyperhydricity, or vitrification, is a physiological malformation affe
cting tissue culture-generated plants. This malformation is associated
with excessive hydration and poor lignification and results in poor r
egeneration of plants. We have tested hyperhydricity prevention in ore
gano by several nonspecific polysaccharide-producing rhizosphere bacte
ria. Among these bacteria, Pseudomonas mucidolens and another Pseudomo
nas sp. prevented hyperhydricity and improved acclimation of oregano c
lones. These two bacteria have more advantages for commercial applicat
ions than Pseudomonas strains isolated previously from oregano.