STRUCTURAL GENE (NIRS) FOR THE CYTOCHROME CD(1) NITRITE REDUCTASE OF ALCALIGENES-EUTROPHUS H16

Authors
REES E SIDDIQUI RA KOSTER F SCHNEIDER B FRIEDRICH B
Citation
E. Rees et al., STRUCTURAL GENE (NIRS) FOR THE CYTOCHROME CD(1) NITRITE REDUCTASE OF ALCALIGENES-EUTROPHUS H16, Applied and environmental microbiology, 63(2), 1997, pp. 800-802
Citations number
25
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
63
Issue
2
Year of publication
1997
Pages
800 - 802
Database
ISI
SICI code
0099-2240(1997)63:2<800:SG(FTC>2.0.ZU;2-V
Abstract
Denitrification by Alcaligenes eutrophus H16 is genetically linked to megaplasmid pHG1. Unexpectedly, the gene encoding the nitrite reductas e (nirS) was identified on chromosomal DNA. The nirS product showed ex tensive homology with periplasmic nitrite reductases of the heme cd(1) -type. Disruption of nirS abolished nitrite-reducing ability, indicati ng that NirS is the enzyme essential for denitrification in A. eutroph us.