EFFECT OF SPERM MOTILITY ON SEPARATION OF BOVINE X-BEARING AND Y-BEARING SPERMATOZOA BY MEANS OF FREE-FLOW ELECTROPHORESIS

Authors
MANGER M BOSTEDT H SCHILL WB MILEHAM AJ
Citation
M. Manger et al., EFFECT OF SPERM MOTILITY ON SEPARATION OF BOVINE X-BEARING AND Y-BEARING SPERMATOZOA BY MEANS OF FREE-FLOW ELECTROPHORESIS, Andrologia, 29(1), 1997, pp. 9-15
Citations number
18
Categorie Soggetti
Andrology
Journal title
Andrologia → ACNP
ISSN journal
03034569
Volume
29
Issue
1
Year of publication
1997
Pages
9 - 15
Database
ISI
SICI code
0303-4569(1997)29:1<9:EOSMOS>2.0.ZU;2-2
Abstract
Free-flow electrophoresis was used on bovine spermatozoa to test the h ypothesis that are surface charge differences between X and Y chromoso me-bearing spermatozoa. Spermatozoa were deflected towards the anode b y means of tromethamine (THAM) buffers of varying concentrations, and were separated into two populations under specific conditions Experime ntal temperature and initial sperm motility had a significant effect o n sperm distribution in the electric field. The results of DNA hybridi zation assays indicated an enrichment for Y chromosome-bearing spermat ozoa.