N. Suzuki et al., PARTIAL CHARACTERIZATION OF A CALCITONIN-LIKE SUBSTANCE FROM THE BROCKMANN BODIES OF THE MEDAKA ORYZIAS-LATIPES, Fisheries science, 61(4), 1995, pp. 687-690
Biochemical natures of calcitonin (CT)-like substance obtained from Br
ockmann bodies (BB) of the medaka Oryzias latipes (Teleostei) were exa
mined. A crude extract of BE was fractionated by reversed-phase HPLC a
nd screened with anti-salmon CT antisetum by Western blotting. Althoug
h 4 fractions showed positive immunoreactivity, CT-like biological act
ivity was detected only in one of these fractions. The molecular weigh
t of the identified CT-like substance was calculated as 20 kDa, which
is considerably higher than that (3.5 kDa) of authentic CTs of other v
ertebrates.