PARTIAL CHARACTERIZATION OF A CALCITONIN-LIKE SUBSTANCE FROM THE BROCKMANN BODIES OF THE MEDAKA ORYZIAS-LATIPES

Authors
SUZUKI N KASE Y SASAYAMA Y KAMBEGAWA A MAGTOON W SRIVASTAV AK
Citation
N. Suzuki et al., PARTIAL CHARACTERIZATION OF A CALCITONIN-LIKE SUBSTANCE FROM THE BROCKMANN BODIES OF THE MEDAKA ORYZIAS-LATIPES, Fisheries science, 61(4), 1995, pp. 687-690
Citations number
20
Categorie Soggetti
Fisheries
Journal title
Fisheries science → ACNP
ISSN journal
09199268
Volume
61
Issue
4
Year of publication
1995
Pages
687 - 690
Database
ISI
SICI code
0919-9268(1995)61:4<687:PCOACS>2.0.ZU;2-2
Abstract
Biochemical natures of calcitonin (CT)-like substance obtained from Br ockmann bodies (BB) of the medaka Oryzias latipes (Teleostei) were exa mined. A crude extract of BE was fractionated by reversed-phase HPLC a nd screened with anti-salmon CT antisetum by Western blotting. Althoug h 4 fractions showed positive immunoreactivity, CT-like biological act ivity was detected only in one of these fractions. The molecular weigh t of the identified CT-like substance was calculated as 20 kDa, which is considerably higher than that (3.5 kDa) of authentic CTs of other v ertebrates.