INTERACTIONS OF L-ASCORBYL-2-PHOSPHATE MG AND OXIDIZED FISH-OIL ON RED-SEA BREAM JUVENILES

A 2 x 3 factorial experiment was conducted using two degrees of oxidat ion of the 10% supplemental fish oils in the diet [fresh oil, peroxide value 17 meg/kg (FO) and oxidized oil, peroxide value 256 meq/kg (XO) ], three levels of supplemental L-ascorbyl-2-phosphate-Mg (APM) 0, 50 and 500 mg/100 g diet on red sea bream Pagrus major juveniles (initial body weight similar to 3.36 g). The fish grew 9-10 fold over a 60 day s period of feeding in the groups of APM-supplemented with fresh or ox idized oil (50-FO, 50-XO, 500-FO and 500-XO) and showed better perform ance (weight gain, feed conversion efficiency, specific growth rate, s urvival), body condition (condition factor, hepatosomatic index, colla gen content of bone), and biochemical parameters (tissue ascorbic acid and thiobarbituric acid value (TEA value) than the groups of APM-unsu pplemented (0-FO and 0-XO). However, the analysis of variance revealed that there was significant (p < 0.05) interaction of APM-level and oi l-type in the tissues ascorbic acid level and condition factor. APM ha d strong main effect on all variables whereas oil type had not. A depl etion of APM from the diets (0-FO and 0-XO) resulted in accelerating l ipid peroxidation of muscle measured as TBA value and low lipid accumu lation as triglyceride in muscle and liver.