ESTABLISHMENT AND CHARACTERIZATION OF A PRONEPHRIC STROMAL CELL-LINE (TPS) FROM RAINBOW-TROUT, ONCORHYNCHUS-MYKISS W

A new stromal adherent cell line, called trout pronephric stroma (TPS) , was initiated from a long-term pronephric culture of an adult rainbo w trout, and subcultured 104 times over a period of 4 years. This stud y describes the culture conditions and characterization by enzyme-cyto chemistry, electron microscopy, isoenzyme profile, cytogenetic techniq ues, and viral susceptibility. The cell types in TPS cultures consiste d of fibroblastic, epithelioid and giant cells. The modal chromosome n umber is 58. The optimum growth temperature of the TPS cells is 18-22 degrees C, with a population doubling time of 27 h. The TPS line is su sceptible to viral haemorrhagic septicaemia (VHS) virus and infectious haemopoietic necrosis (IHN) virus. (C) 1995 Academic Press Limited