D. Pillay et al., ELECTROPHORETIC AND IMMUNOLOGICAL ANALYSIS OF LIPOPOLYSACCHARIDES OF XANTHOMONAS-ALBILINEANS FROM 3 GEOGRAPHICAL REGIONS, Letters in applied microbiology, 21(3), 1995, pp. 210-214
Lipopolysaccharides (LPS) were extracted from the type species of the
sugarcane leaf scald pathogen Xanthomonas albilineans from Fiji and ad
ditional isolates from Australia, Mauritius and South Africa. After re
solution by sodium dodecyl sulphate-polyacrylamide gel electrophoresis
, the LPS were visualized using a modified silver staining method and
analysed by densitometric scanning. Three distinct electrophoretic pat
terns were identified: the LPS pattern of the Fijian isolate differed
from those of the other isolates. Immunological cross-reactivity of th
e LPS and gamma globulins was assessed by immunoblotting. Immunoblotti
ng showed that although IgG raised against other isolates cross-reacte
d with the LPS of one another, they did not react with the purified LP
S of the Fijian isolate. Differences in LPS patterns and serological c
ross-reactivity may be due to differences in the length and conformati
on of the O-polysaccharide chains of the LPS.