ANALYSIS OF MONOSACCHARIDE AND OLIGOSACCHARIDE ISOMERS DERIVATIZED WITH 9-AMINOPYRENE-L,4,6-TRISULFONATE BY CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE

A method of analysis of isomers of mono- and oligosaccharides derivati zed with 9-aminopyrene-1,4,6-trisulfonate (APTS) by capillary electrop horesis (CE) in simple buffer systems is presented. The derivatization chemistry is performed at 75 degrees C for 1 h for most mono- and oli gosaccharides. Sialyllactose was derivatized at 37 degrees C for 15 h without significant desialylation. Most of the APTS derivatized isomer s of mono and oligosaccharides are resolved in buffers such as acetate (pH 5.0), 3-(N-morpholino)-propanesulfonic acid (pH 7.0), and phospha te (pH 7.4). The mechanism of CE separation using the above buffers is based on the differences in hydrodynamic volume of the derivatized sp ecies and is different from that in berate or high pH buffer, (C) 1995 Academic Press, Inc.