ANALYTICAL VALIDATION OF A GENERAL PROTOCOL FOR THE PREPARATION OF DOSE-CONTROLLED SOLUTIONS IN ALUMINUM TOXICOLOGY

Aluminium(III) solutions suitable fbr careful toxicological investigat ion both in vivo and in vitro, are prepared from AlCl3 . 6H(2)O and fr om neutral metal complexes, Al(lactate)(3), Al-2(citrate)(2)(H2O)(6), Al(acetylacetonate)(3) and Al(maltolate)(3), by dissolving the pure co mpounds in TRIS-HCI buffer solutions. The metal concentrations in the supernatant liquids are determined spectrophotometrically under reprod ucible conditions. Al(acetylacetonate)(3) and Al(maltolate)(3) are det ected as molecular entities by using H-1 NMR spectroscopy down to conc entrations of 200 and 100 mu mol 1(-1), respectively.