CHROMATOGRAPHIC BEHAVIOR OF DEOXYRIBONUCLEOSIDES WITH RESPECT TO ORGANIC MODIFIER CONTENT IN THE MOBILE-PHASE

A chromatographic behaviour of five deoxyribonucleosides (dCyd, dUrd, dGuo, dThd, and dAdo) has been studied under isocratic conditions of r eversed-phase HPLC. The Waters column packed by 10 mu m mu-Bondapak C1 8 was used. The volume fraction (F) of organic modifier was changed fr om 0.05 to 0.30, and to 0.12 for methanol and acetonitrile, respective ly. Furthermore, the capacity factors with a mobile phase of 100 % wat er (k'(w)) have been measured. Linear regression has been carried out for the log k' vs. F data and the intercepts and the slopes of these p lots were calculated. The slope magnitudes of different solutes were a lmost the same for each organic modifier. In all cases, the experiment al values of k'(w) were higher than extrapolated ones. For each solute , extrapolation of log k' vs. F gives practically the same intercept v alues for both organic modifiers. It was concluded that the intercepts calculated in the region of small content of organic modifier are ind ependent on the nature of organic modifier and correlate with deoxyrib onucleosides nature only.