A novel rapid method for the identification of colonies of Clostridium
perfringens (key iD Lab M Ltd. Bury, UK) was evaluated. The method co
nsists of a test strip containing substrates for pre-formed enzymes se
lected for optimum differentiation of C. perfringens from other clostr
idia. One hundred and forty-six strains of clostridia were tested usin
g the key iD strip. The strip successfully confirmed the identity of a
ll 73 strains of C. perfringens tested, and differentiated these from
73 strains of 20 other clostridial species. C. absonum and C. baratii,
species which are very similar to C. perfringens, could also be diffe
rentiated by this method. The key iD strip is recommended for laborato
ries as a rapid alternative to more conventional tests for presumptive
identification of C. perfringens.