STRUCTURAL EVALUATION OF HUMAN AND SHEEP BONE AND COMPARISON WITH SYNTHETIC HYDROXYAPATITE BY FT-RAMAN SPECTROSCOPY

The composition of whole human and sheep cortical bone tissue, and of a synthetic hydroxyapatite (P120), were compared using Fourier transfo rm Raman (FT-Raman) spectroscopy. Deproteination procedures to remove the hulk of the collagen present in bone tissue allowed isolation of t he mineral phase. A comparison of the spectra obtained from both whole and deproteinated bone with those of synthetic hydroxyapatite showed direct correlation only in the region of 952 cm(-1) (symmetric P-O mod e). In contrast, human and sheep bone were very closely matched in bot h, the organic and inorganic structures. The results demonstrate that deproteination of bone is not a necessary precursor to obtain spectral information. (C) 1995 John Wiley & Sons, Inc.