K. Hansen et al., IN-VITRO SCREENING OF TRADITIONAL MEDICINES FOR ANTIHYPERTENSIVE EFFECT BASED ON INHIBITION OF THE ANGIOTENSIN-CONVERTING ENZYME (ACE), Journal of ethnopharmacology, 48(1), 1995, pp. 43-51
Traditional medicines reported to be used as anti-hypertensives or diu
retics from different regions in the world (China, India and South Ame
rica) have been investigated. The bioassay is based on inhibition of A
CE, as measured from the enzymatic cleavage of the chromophore-fluorop
hore-labelled substrate dansyltriglycine into dansylglycine and diglyc
ine. In total, 31 species have been investigated and the crude extract
s from seven species inhibit the enzyme by more than 50%.