A laser-based Raman microprobe/confocal microscope has been used to pr
ovide structural information on the constituent proteins in several ke
ratins including wool, human hair, and feathers. Depth-profiling studi
es have revealed differences between the outer (cuticle) and inner (co
rtex) regions of human hair fibers with respect to the molecular orien
tation and chemical composition of the constituent proteins. Raman mic
roprobe measurements are applied to the identification and distributio
n analysis of textile auxiliaries on wool fibers. The advantages and u
tility of the Raman microprobe technique for investigating single text
ile fibers are also discussed.