J. Lombardi et Cr. Graham, THE USE OF C-13-MAGNETIC RESONANCE SPECTROSCOPY IN METABOLIC RESEARCH, Journal of nutritional biochemistry, 6(10), 1995, pp. 516-521
Magnetic resonance spectroscopy (MRS) is a powerful investigative tool
that has rapidly become accepted as a valuable means of assessing met
abolic phenomena. C-13 MRS provides a rapid and nondestructive means o
f studying metabolism in intact tissues, organs, and whole organisms.
A major feature of the technique is the ability to follow metabolism n
oninvasively and nondestructively. Benefits of a wide spectral window
for C-13 (>200 ppm), makes C-13 MRS ideal for obtaining semiquantitati
ve information about enriched substrates and their intermediates withi
n living tissues in real time. The increased commercial availability o
f a wide range of C-13-enriched biomolecules combined with the develop
ment of more sensitive instrumentation has made it possible to begin e
xploiting the full potential of C-13 magnetic resonance spectroscopy i
n metabolic research. This review is intended to introduce renders to
the application of C-13 magnetic resonance spectroscopy to selected pr
oblems in metabolic research.