PROTEIN-KINASE INHIBITORS DISRUPT MEMORY FORMATION IN 2 CHICK BRAIN-REGIONS

The amnesic effects of protein kinase inhibitors (H-7, HA-156, TFP, W- 9, and W-13) on memory formation for a one trial peck-avoidance task i n chicks were investigated with bilateral and unilateral injections in to either the left or the right intermediate medial hyperstriatum vent rale (IMHV) or the left or right lobus parolfactorius (LPO). All five inhibitors injected bilaterally 5 min pretraining into either the IMHV or LPO or unilaterally into the left IMHV produced amnesia. Unilatera l injections into the right IMHV did not produce amnesia. Unilateral i njections of W-9 or W-13 into the left but not the right LPO produced amnesia. H-7, HA-156, or TFP did not produce amnesia when injected uni laterally into either the left or right LPO. The time of onset of amne sia produced by injecting TFP and W-13 into the LPO occurred 45 min af ter training, whereas H-7 produced amnesia significantly later (90 min after training). Amnesia induced by TFP, W-13, and H-7 injected into the LPO occurred significantly later than amnesia produced when these agents were injected into the IMHV. Together these data suggest that t he IMHV and LPO process memory sequentially using various protein kina se activities.