QUANTITY OF SUCROSE ALTERS THE TISSUE PATTERN AND TIME-COURSE OF INSULIN-RESISTANCE IN YOUNG-RATS

To determine the effects of the amount of sucrose in the diet on insul in-stimulated glucose metabolism, euglycemic hyperinsulinemic clamps w ere performed on male Wistar rats after one of the following dietary t reatments (n = 6-8/treatment): 1) high-starch diet (68% of total energ y) for 8 wk (ST8), 16 wk (ST16), or 30 wk (ST30); 2) high-sucrose diet (68% of total energy) for 8 wk (SU8), 16 wk (SU16), or 30 wk (SU30); or 3) low-sucrose diet (18% of total energy) for 8 wk (SUL8), 16 wk (S UL16), or 30 wk (SUL30). Body weights were similar in starch- and sucr ose-fed rats at 8 wk (502 +/- 9 g), 16 wk (563 +/- 10 g), and 30 wk (6 07 +/- 26 g). The glucose infusion rate (mu mol . g(-1) . min(-1)) req uired to maintain similar glycemia during clamps was 73.1 +/- 8.8 in S T8, 29.7 +/- 4.9 in SU8 (P < 0.05 vs. ST8 and SUL8), and 76.4 +/- 8.2 in SUL8; 69.9 +/- 8.1 in ST16, 35.1 +/- 5.1 in SU16 (P < 0.05 vs. ST16 and SUL16), and 63.2 +/- 6.5 in SUL16; and 65.4 +/- 7.7 in ST30, 26.0 +/- 5.3 (P < 0.05 vs. ST30), and 36.3 +/- 6.0 in SUL30 (P < 0.05 vs. ST30). Impaired suppression of hepatic glucose production accounted fo r 43, 39, and 34% of the decrease in the glucose infusion rate in SU8 compared with ST8, SU16 compared with ST16, and SU30 compared with ST3 0, respectively, but 78% in SUL30 compared with ST30. These results su ggest that both high- and low-sucrose diets can produce insulin resist ance in young rats. However, the quantity of sucrose in the diet alter s both the time course of induction and tissue distribution of insulin resistance.