F. Liu et al., ANTITUMOR COMPONENTS OF THE CULTURE FILTRATES FROM TRICHOLOMA SP, World journal of microbiology & biotechnology, 11(5), 1995, pp. 486-490
The anti-tumour component (Fraction A) found in the culture filtrates
of a local mushroom, Tricholoma sp. (strain STC20-T1), was purified us
ing DEAE-cellulose ion exchange chromatography and Sepharose CL-4B gel
filtration. Of its two purified sub-fractions, A-1 and A-2, only the
latter showed anti-tumour activity. When Fraction A-2 was treated with
cetyltrimethylammonium hydroxide and chloroform/butanol (24:1, v/v),
it behaved as a protein-bound polysaccharide, with a molecular mass es
timated to be 154 kDa by gel filtration. It contained 40% polysacchari
de, which consisted of fucose, galactose, glucose, arabinose and manno
se, and 30.5% protein, the amino-acid composition of which was determi
ned. Fraction A-2 could inhibit the growth of Sarcoma-180 implanted in
mice intraperitoneally and subcutaneously, with no sign of toxicity,
at a dose of 20 mg/kg.day for 10 days.