PURIFICATION AND PROPERTIES OF A GLUCOSE-FORMING AMYLASE OF LACTOBACILLUS-BREVIS

Authors
ILORI MO AMUND OO OMIDIJI O
Citation
Mo. Ilori et al., PURIFICATION AND PROPERTIES OF A GLUCOSE-FORMING AMYLASE OF LACTOBACILLUS-BREVIS, World journal of microbiology & biotechnology, 11(5), 1995, pp. 595-596
Citations number
6
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
World journal of microbiology & biotechnology → ACNP
ISSN journal
09593993
Volume
11
Issue
5
Year of publication
1995
Pages
595 - 596
Database
ISI
SICI code
0959-3993(1995)11:5<595:PAPOAG>2.0.ZU;2-K
Abstract
An extracellular glucose-forming amylase was produced by Lactobacillus brevis isolated from 'Kagasok tea'. The enzyme was purified 70-fold a nd had optimal activity at 55 degrees C and pH 6.5. Its K-m value for starch was 0.27 mg ml(-1) and its M(r) was approx. 75,900 Da. The acti vity of the enzyme was enhanced by Ca2+, Mg2+, Na+ or K+ and inhibited by EDTA, KCN, citric acid and L-cysteine.